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Analytical Laboratory I




Facility Location
Cuajimalpa Campus
Division of Natural Sciences and Engineering

8th Floor, L-854

Contact Information
Dr. José Campos Terán
jcampos@cua.uam.mx

Additional Details

• National Laboratory Affiliation
• Collaboration agreement

Introduction

This facility is part of the National Multidisciplinary Laboratory dedicated to the characterization of materials and nanostructures.

The Analytical Laboratory I (L‑854) is a facility of the Division of Natural Sciences and Engineering at the Cuajimalpa Campus of the Universidad Autónoma Metropolitana. This facility focuses on the qualitative and quantitative characterization of organic and inorganic compounds using high-resolution, high-precision, and high-sensitivity analytical techniques. Its primary role involves providing scientific and technical support to research projects, technological development, academic innovation, and professional training in fields such as chemistry, biotechnology, pharmacology, environmental science, food engineering, and materials science.

• Academic Technical Coordinator:
Dr. Juan Gabriel Vigueras Ramírez

jvigueras@cua.uam.mx

The laboratory is equipped with a comprehensive range of instruments that support a variety of analytical methodologies. Key equipment includes high-performance liquid chromatography systems (HPLC) in binary and quaternary configurations, gas chromatographs coupled with selective detectors (GC-ECD and GC-MS), UV-Visible spectrophotometers with temperature control, CHNS-O elemental analyzers, total organic carbon (TOC) systems, 96-well ELISA plate readers, and automated biochemical analyzers. This technological diversity enables researchers to work with complex sample matrices such as plant extracts, soils, food products, wastewater, pharmaceuticals, fermentation broths, polymers, and biological materials.

The lab’s analytical capabilities encompass the separation and quantification of active compounds, metabolites, pigments, organic acids, and proteins, as well as the detection of contaminants such as pesticides, residual solvents, and halogenated compounds. It also performs elemental analysis to determine carbon, hydrogen, nitrogen, sulfur, and oxygen content in biomass, fertilizers, industrial products, and synthetic materials. Researchers rely on the lab to support studies in biodegradation, quality control, biotechnological process efficiency, and environmental impact assessment. The team implements methods validated under international standards to ensure data traceability, reproducibility, and quality.

The laboratory follows a shared-use model, serving both internal academic staff and external researchers through institutional agreements or collaborations. It has provided analytical services to various divisions within the university, such as the Division of Natural Sciences and Engineering (DCNI) and the Biological and Health Sciences Divisions (DCBS) at the Azcapotzalco and Iztapalapa campuses. Collaborations also extend to institutions like the National Autonomous University of Mexico (UNAM), the National Polytechnic Institute (IPN), and other public universities. The lab contributes to interinstitutional projects that require analytical validation, experimental data generation, or methodological design.

One of the lab’s greatest strengths lies in its affiliation with the National Multidisciplinary Laboratory for Materials and Nanostructure Characterization, a national infrastructure recognized within the National System of Public Research Centers, coordinated by the Secretariat of Science, Humanities, Technology, and Innovation (SECIHTI). This affiliation lends the laboratory strategic national relevance by connecting it to collaborative research networks, promoting open access to scientific infrastructure, and amplifying the social impact of the knowledge it generates. Participation in this network also drives continuous updates to the lab’s technological and methodological capabilities.

Analytical Laboratory I stands out for its rigorous approach to analytical methodology, commitment to traceable and validated results, and strict adherence to national and international standards.

Projects and Activities


The Analytical Laboratory I plays a strategic role within the scientific and technological ecosystem of the Universidad Autónoma Metropolitana. It provides technical and methodological support for research projects, academic training, and specialized analytical services. The lab operates under a project-based framework, where each analytical activity follows a structured process that ensures traceability of results, method validation, and alignment with scientific goals.

One of the lab’s core functions involves technical support for basic and applied research projects —both individual and multidisciplinary— that rely on advanced analytical techniques to characterize complex samples. These projects focus on topics such as the synthesis and evaluation of functional materials, the detection of emerging contaminants, the profiling of secondary metabolites in plant extracts, the quality assessment of foods and fermented beverages, and the validation of biotechnological processes with environmental or health relevance. Laboratory staff participate in every phase of these projects, from experimental planning to data generation, interpretation, and preparation of technical reports and scientific publications.

The lab also supports undergraduate capstone projects, thesis work at the master’s and doctoral levels, and academic research stays. Students from the Division of Natural Sciences and Engineering (DCNI) receive training in equipment operation, sample preparation, and critical data interpretation. This training process builds not only technical skills but also a professional, collaborative mindset grounded in institutional protocols, experimental logs, and method validation practices.

In addition to academic use, the lab offers analytical services to third parties, both within and outside the university. Faculty, researchers, and external institutions can request analytical support for sample characterization under defined parameters. Available services include compound quantification via HPLC, volatile substance identification via GC-MS, total organic carbon determination, UV-Vis spectrophotometry, ELISA-style colorimetric assays, and elemental analysis (CHNS-O), among others. All external service projects follow formal documentation processes, including service requests, work logs, internal validations, and certified result delivery.

Preliminary Characterization Services

Analytical Laboratory I provides exploratory analytical services without official certification, designed to support preliminary studies, sample screening, and the generation of indicative data. Research groups and external collaborators use these services to gain an initial understanding of the composition, quality, or presence of target compounds in their samples. The lab applies advanced instrumentation, internally validated methods, and controlled conditions to ensure reliability.

These preliminary characterizations prove especially valuable in projects with large sample volumes. By applying technical selection criteria, users can narrow down broad sample sets to those with analytical relevance and then submit the selected samples for certified testing elsewhere. This strategy supports informed decision-making, optimizes resources, and significantly reduces costs associated with high-level analytical testing.

The lab can perform preliminary quantification of active compounds, contaminant screening, chromatographic profiling, UV-Vis absorbance spectra, estimation of total organic carbon concentrations, and determination of indicative elemental composition (CHNS-O). Each result includes a technical summary detailing the equipment used, analytical conditions, and relevant observations.

The lab also contributes to technology development and process optimization efforts. These include testing alternative analytical methods, deploying faster or more sensitive techniques, designing automated routines, and integrating new detection systems. Faculty from multiple disciplines collaborate on these efforts, ensuring the lab stays responsive to emerging research and regulatory demands.

Another key function involves generating data for institutional monitoring and surveillance protocols. The lab supports projects focused on water quality evaluation, compound tracking in environmental campaigns, and biomarker studies in clinical or preclinical contexts. These efforts reinforce the lab’s impact in public health, environmental sustainability, and applied science.
Key Equipment

Mass Selective Detector Coupled to Gas Chromatograph (117595). Model: Agilent 5975B VL with GC 6890N. This chromatographic system integrates an Agilent 6890N gas chromatograph with a 5975B VL mass selective detector, creating a robust platform for identifying volatile and semi-volatile organic compounds. The workstation includes specialized mass spectrometry software that enables structural analysis through spectral library matching. Researchers use this system to investigate trace compounds, emerging contaminants, solvents, pesticides, pharmaceuticals, and metabolites. At Analytical Laboratory I, it supports environmental and pharmaceutical research, as well as the development of analytical methods for complex sample matrices.

High-Performance Liquid Chromatograph -HPLC (175297). Model: Waters ARC HPLC System. This modular HPLC system features a core pump unit, column oven, both manual and automatic injectors, and three detectors: a photodiode array UV-Vis detector (PDA 2998), a refractive index detector (RI 2414). It includes Empower 3 multi-user software, a DELL workstation and Symmetry C18 analytical columns. This setup enables multicomponent analysis of natural products, food, fermented samples, pharmaceuticals, and organic compounds of environmental or biomedical interest. At Analytical Laboratory I, it plays a key role in quantifying water-soluble organic compounds, profiling metabolic pathways, and validation of analytical methods.

Total Organic Carbon Analyzer - TOC (117261). Model: Shimadzu TOC-LCSH with ASI-L and SSM-5000A. This system measures total organic carbon (TOC), inorganic carbon (IC), and total nitrogen (TN) in both liquid and solid samples. It uses high-temperature catalytic oxidation at 680°C and non-dispersive infrared detection, offering high sensitivity and precision. The instrument complies with EPA, ASTM, and international pharmacopoeia standards. It features an autosampler for 63 vials and a solid sample module (SSM) for direct analysis of sludges, powders, or biomass. At Analytical Laboratory I, the TOC analyzer supports studies in water quality, pharmaceutical cleaning validation, organic waste characterization, and monitoring of industrial, domestic, and lab-grade water. Its versatility makes it a critical tool for environmental and bioprocess research

Available Technologies

Analytical Laboratory I features a highly specialized set of instruments, organized into workstations according to analytical techniques. The team designed the space to ensure traceable analyses, user safety, and efficient project development. The laboratory operates within a regulated electrical network, features localized ventilation, gas supply lines, and purified water systems, as well as purpose-built furniture for both wet and dry methods. The layout separates areas by function—sample preparation, injection, analysis, documentation, and cleaning—allowing for safe and organized workflows.

In the liquid chromatography area, the lab operates three high-capacity HPLC systems. The team utilizes a binary VARIAN ProStar system (118143) equipped with an autosampler, column oven, and three detectors: a dual UV-Vis detector, a refractive index (RI) detector, and an evaporative light scattering (ELSD) detector, all controlled through Galaxie software. This platform enables qualitative and quantitative analysis of sugars, alcohols, organic acids, inorganic ions, pigments, metabolites, and proteins. A second system, the quaternary Knauer SmartLine HPLC (118206), also features an autosampler and oven, and integrates conductivity, four-channel UV-Vis, and photodiode array (PDA) detectors, which are managed with ClarityChrom. Researchers use this configuration extensively to analyze pigments, aromatic compounds, and biomolecules. The third setup, a Waters ARC HPLC (175297), features PDA and RI detectors and operates with Empower 3 software to facilitate targeted analysis of amino acids, phytohormones, and carbohydrates across a broad range of sample types.

In gas chromatography, the lab operates two key systems. The first combines an Agilent 6890 gas chromatograph with a 5975B MSD mass spectrometer (117595), running on ChemStation software with spectral libraries. This setup is ideal for analyzing pesticides, pharmaceuticals, and trace compounds up to 700 Da in environmental, food, or pharmaceutical matrices. The second system is a VARIAN Star 3400 CX GC (117543) fitted with an electron capture detector (ECD) and operated through ClarityChrom, primarily used for halogenated compounds in agricultural or environmental samples.

For UV-Vis spectrophotometry, the lab features two complementary instruments. The Thermo Scientific Evolution 300 (117582) features temperature control and VisionPro software for colorimetric, kinetic, and spectral scanning methods. The Genesys 150 from the same brand (169640) provides high-precision absorbance spectra for standard lab assays. Both are essential for compound identification and quantification in the UV-visible range.

The lab also supports high-throughput analysis using microplate technology. The Daigger ELISA plate reader (118018) is used for colorimetric assays in 96-well plates, primarily for biochemical and immunoenzymatic applications. The YSI 2900 biochemical analyzer (117260) complements this setup with automated quantification of glucose, xylose, ethanol, and other key metabolites, using direct injection or plate formats—vital for fermentation and bioprocess studies.

In elemental analysis, the lab relies on a Thermo Flash 2000 analyzer (900220121510), paired with an autosampler and a Mettler Toledo microbalance, to determine carbon, hydrogen, nitrogen, sulfur, and oxygen (CHNS-O) in solid samples. Researchers use this system to characterize biomass, food, polymers, fertilizers, soil, and solid waste.

For total organic carbon (TOC) analysis, the Shimadzu TOC-LCSH system (117261) offers high-precision determination of total carbon, organic carbon, inorganic carbon, and total nitrogen in liquid and solid samples. It features an ASI-L autosampler for liquids and an SSM-5000A module for solids. Operating through high-temperature catalytic oxidation and infrared detection, this instrument plays a key role in water quality studies, cleaning validation, and the analysis of complex matrices.

All instruments are connected to dedicated workstations with specialized software, technical monitoring, usage logs, and scheduled maintenance. The lab adheres to a shared-use policy, with academic and technical oversight, to ensure safe, reproducible, and high-quality operations. This advanced infrastructure supports multidisciplinary research, graduate-level academic training, and specialized analytical services for both internal and external users.

Primary User Groups


Analytical Laboratory I serves a diverse academic community that includes internal academic staff, students in training, national researchers, and external institutions. The lab follows a shared-use policy that promotes access to highly specialized scientific infrastructure, fostering multidisciplinary collaboration, academic development, and institutional partnerships.

At the Universidad Autónoma Metropolitana (UAM), faculty and students from the Division of Natural Sciences and Engineering (DCNI), the Department of Processes and Technology (DPT), and the Department of Natural Sciences (DCN) frequently use the lab. Faculty and researchers from other divisions, such as Biological and Health Sciences (DCBS), also rely on the lab’s capabilities. Academic staff from other UAM campuses—especially Iztapalapa and Azcapotzalco—regularly access the lab’s equipment to support collaborative projects, generate analytical data, validate methods, and receive specialized training.

Outside the institution, the lab maintains active collaboration with national universities and public research centers, including the National Autonomous University of Mexico (UNAM), the National Polytechnic Institute (IPN), and several state universities and public laboratories. These collaborations take shape through service agreements, joint research efforts, and participation in projects funded at the federal and local levels.

From a training perspective, undergraduate students use the lab’s equipment and methodologies for capstone projects, research stays, and social service. Graduate students at the specialization, master’s, doctoral, and postdoctoral levels also work in the lab. The lab team provides technical training in instrument handling, data interpretation, and best analytical practices, preparing users for high-level research environments that mirror professional settings.

By participating in the National Multidisciplinary Laboratory for Materials and Nanostructures Characterization, recognized within the National System of Public Research Centers and coordinated by the Secretariat of Science, Humanities, Technology, and Innovation (SECIHTI), the lab has expanded its reach nationwide. This strategic affiliation connects the lab to interinstitutional research networks, encourages open access to critical infrastructure, and supports continuous updates to its technological and methodological capabilities.

Access Policies and Guidelines


Access to Analytical Laboratory I follows strict operational safety standards, technical responsibility, and prior training requirements to protect both users and equipment. These policies aim to support a safe, organized, and regulation-compliant working environment.

The lab opens Monday through Friday from 8:00 a.m. to 4:00 p.m. Anyone needing access outside those hours must request written approval from the lab coordinator in advance. The lab does not allow after-hours access without supervision or a clear assessment of operational risks.

Only academic staff, technical personnel, and students actively involved in approved research projects or training programs may enter the lab. The lab coordinator reviews and approves each user’s registration. Individuals not affiliated with academic or service-related tasks may not access the facility.

All users must complete a mandatory technical orientation before working independently. This training covers safety procedures, equipment handling, lab protocols, and station-specific rules. Users must renew this training yearly or whenever the lab incorporates new equipment or techniques. Failure to complete training or follow rules results in immediate loss of lab access.

During lab work, users wear appropriate personal protective equipment (PPE), including a lab coat, chemical-resistant gloves, safety goggles, and, when necessary, a mask or additional protection. The lab strictly forbids food, drinks, headphones, or non-essential items in analysis areas. Each user must keep their station clean, record all activity in the lab log, and report spills, malfunctions, or any incidents directly to the technical team.

Everyone who uses the lab commits to upholding standards of safety, quality, and shared responsibility. Any negligent actions, equipment misuse, or policy violations will result in disciplinary measures ranging from temporary suspension to permanent exclusion, as determined by the lab coordinator and department leadership.

These access policies reflect the laboratory’s commitment to a professional, collaborative, and accountable research environment.
Information Provided by the Division of Natural Sciences and Engineering



Universidad Autónoma Metropolitana, 2025.